Goat Anti-TCF3 / ITF1 Antibody (OAEB02356)

Catalog No: OAEB02356 (Formerly GWB-945916)

Size:100UG

OAEB02356 (0.01ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02356 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Additional Information: Reactivity Info: Tested: Human; Expected: Human
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: TCF3
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 78%; Human: 100%; Mouse: 85%; Pig: 92%; Rat: 85%
• Peptide Sequence: C-KAPRARTSPDEDED
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:128000.
  Western Blot: Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). Recommended concentration: 0.01-0.03ug/ml.
  Immunohistochemistry: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5ug/ml.
• Reference: * Greenbaum S, Lazorchak AS, Zhuang Y.; Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes.; J Biol Chem. 2004 Oct 22;279(43):45028-35.; Pubmed 15310760

Target Info

• Gene Symbol: TCF3
• Gene Full Name: transcription factor 3
• Alias Symbols: AGM8;bHLHb21;class B basic helix-loop-helix protein 21;E2A;E2A-HLF fusion transcript protein;E47;helix-loop-helix protein HE47;immunoglobulin transcription factor 1;ITF1;kappa-E2-binding factor;negative vitamin D response element-binding protein;NOL1-TCF3 fusion;p75;TCF-3;transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47);transcription factor 3 variant 3;transcription factor E2-alpha;transcription factor ITF-1;VDIR;VDR interacting repressor;vitamin D receptor-interacting repressor.
• NCBI Gene Id: 6929
• Protein Name: Transcription factor E2-alpha
• Uniprot ID: P15923
• Protein Accession #: NP_003191.1
• Molecular Weight: 67.6