Goat Anti-SPA1 / SIPA1 Antibody (OAEB01861)

Catalog No: OAEB01861 (Formerly GWB-C3F580)

Size:100UG

OAEB01861 staining (1ug/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Reactivity Info: Tested: Human; Expected: Human
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: SIPA1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%; Rat: 92%
• Peptide Sequence: C-ASKQLGSPTADLA
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272(44):28081-8; PMID: 9346962). Recommended concentration: 1-3ug/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.
• Reference: * Hattori M, Tsukamoto N, Nur-e-Kamal MS, Rubinfeld B, Iwai K, Kubota H, Maruta H, Minato N.; Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression.; Mol Cell Biol. 1995 Jan;15(1):552-60.; Pubmed 7799964

Target Info

• Gene Symbol: SIPA1
• Gene Full Name: signal-induced proliferation-associated 1
• Alias Symbols: GTPase-activating protein Spa-1;p130 SPA-1;signal-induced proliferation-associated gene 1;signal-induced proliferation-associated protein 1;sipa-1;SPA1.
• NCBI Gene Id: 6494
• Protein Name: Signal-induced proliferation-associated protein 1
• Uniprot ID: Q96FS4
• Protein Accession #: NP_694985.28
• Molecular Weight: 112