Goat Anti-PTBP1 / PTB Antibody (OAEB02478)

Catalog No: OAEB02478 (Formerly GWB-520E7D)

Size:100UG

OAEB02478 (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02478 (5ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: PTBP1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 84%
• Peptide Sequence: DGIVPDIAVGTKR-C
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:8000.
  Western Blot: Approx 55-60 kDa band observed in lysates of cell line Jurkat (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 1-3ug/ml.
  Immunohistochemistry: In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 5-10ug/ml.
• Reference: * Galban S, Kuwano Y, Pullmann R Jr, Martindale JL, Kim HH, Lal A, Abdelmohsen K, Yang X, Dang Y, Liu JO, Lewis SM, Holcik M, Gorospe M.; RNA-binding proteins HuR and PTB promote the translation of hypoxia-inducible factor 1alpha.; Mol Cell Biol. 2008 Jan;28(1):93-107.; Pubmed 17967866
  * Charlet-B N, Logan P, Singh G, Cooper TA.; Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing.; Mol Cell. 2002 Mar;9(3):649-58.; Pubmed 11931771

Target Info

• Gene Symbol: Ptbp1
• Gene Full Name: polypyrimidine tract binding protein 1
• Alias Symbols: AA407203;AL033359;D12Mgi4;heterogeneous nuclear ribonucleoprotein I;hnRNP I;HNRPI;polypyrimidine tract-binding protein 1;pPTB;Pt;Ptb;PTB1;PTB-1;PTB2;PTB3;PTB4;Pybp;Pybp1;Pybp2;pyrimidine binding protein 1;pyrimidine binding protein 2.
• NCBI Gene Id: 19205|29497
• Protein Name: Polypyrimidine tract-binding protein 1
• Uniprot ID: Q9BUQ0
• Protein Accession #: NP_002810.1
• Molecular Weight: 59.6, 59.0, 57.2; 21.9