Goat Anti-Psoriasin / S100A7 Antibody (OAEB01001)

Catalog No: OAEB01001 (Formerly GWB-C43329)

Size:100UG

OAEB01001 (0.1ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human S100A7 with C-terminal MYC tag probed with OAEB01001 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with OAEB01001 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Amino Acids: 101 aa
  Reactivity Info: Tested: Human; Expected: Human
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: S100A7
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Peptide Sequence: C-HKYTRRDDKIDKP
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:32000.
  Western Blot: Approx 15kDa band observed in Human Skin lysates (calculated MW of 11.5kDa according to NP_002954.1). Recommended concentration: 1-3ug/ml. In transfected HEK293 transiently expressing full-length Human S100A7 (myc and DYKDDDDK tagged), a band of approx. 16kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5ug/ml. Approx 15kDa band was also observed in Human Skin lysates (calculated MW of 11.5kDa according to NP_002954.1). Recommended concentration: 0.3-1ug/ml.
• Reference: Webb M, Emberley ED, Lizardo M, Alowami S, Qing G, Alfia\1ar A, Snell-Curtis LJ, Niu Y, Civetta A, Myal Y, Shiu R, Murphy LC, Watson PH.
  Expression analysis of the mouse S100A7/psoriasin gene in skin inflammation and mammary tumorigenesis.
  BMC Cancer. 2005 Feb 17;5(1):17. PMID: 15717926

Target Info

• Gene Symbol: S100A7
• Gene Full Name: S100 calcium binding protein A7
• Alias Symbols: protein S100-A7;PSOR1;psoriasin 1;S100A7c.
• NCBI Gene Id: 6278
• Protein Name: Protein S100-A7
• Uniprot ID: P31151
• Protein Accession #: NP_002954.1
• Molecular Weight: 11.5