Goat Anti-PIWI / HIWI Antibody (OAEB00716)

Catalog No: OAEB00716 (Formerly GWB-6A5100)

Size:100UG

OAEB00716 (4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: PIWIL1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat, Dog, Pig
• Peptide Sequence: C-SNRKDKYDAIKKY
• Concentration: 0.5 mg/ml
• Specificity: This antibody is expected to recognize both reported isoforms (NP_004755.2; NP_001177900.1).
• Application Info: ELISA: antibody detection limit dilution 1:64000
  WB: Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 98.6kDa according to NP_004755.2. All detected bands were successfully blocked by incubation with the immunizing peptide.
  IHC: In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration: 4-6 ug/ml.
  

Target Info

• Gene Symbol: Piwil1|PIWIL1
• Gene Full Name: piwi like RNA-mediated gene silencing 1|piwi-like RNA-mediated gene silencing 1
• Alias Symbols: CT80.1;HIWI;MI;MIWI;PIWI;piwi homolog;piwi-like homolog 1;piwi-like protein 1.
• NCBI Gene Id: 57749|9271
• Protein Name: Piwi-like protein 1
• Uniprot ID: Q96J94
• Protein Accession #: NP_004755.2
• Molecular Weight: 98.6; 94.8