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| Datasheets/Manuals | Printable datasheet for Goat Anti-OCT4 / POU5F1 (mouse) Antibody (OAEB00964) |
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| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | WB |
| Additional Information | Epitope Info: Internal region Immunogen: Peptide with sequence C-DRPNAVKLEKVEP, from the internal region of the protein sequence according to NP_038661.2. |
| :: | Reactivity Info: Tested: Mouse; Expected: Mouse, Rat |
| Reconstitution and Storage | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | POU5F1 |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Rat: 91% |
| Peptide Sequence | C-DRPNAVKLEKVEP |
| Concentration | 0.5 mg/ml |
| Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Application Info | Peptide ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approx 40kDa band observed in lysates of cell line NIH3T3 (calculated MW of 38.2kDa according to NP_038661.2). Recommended concentration: 1-3ug/ml. |
| Reference | Liu TM, Wu YN, Guo XM, Hui JH, Lee EH, Lim B, ; Effects of ectopic Nanog and Oct4 overexpression on mesenchymal stem cells.; Stem cells and development 2009 Sep 18 (7): 1013-22.; PMID: 19102659 |
| Gene Symbol | Pou5f1 |
|---|---|
| Gene Full Name | POU domain, class 5, transcription factor 1 |
| Alias Symbols | NF-A3;Oct;Oct-;Oct3;Oct-3;Oct3/;Oct-3/;Oct3/4;Oct-3/4;Oct4;Oct-4;octamer-binding protein 3;octamer-binding protein 4;octamer-binding transcription factor 3;octamer-binding transcription factor 4 alternative;Otf;Otf-;Otf3;Otf3-;Otf-3;Otf3g;Otf3-rs7;Otf4;Otf-4;POU domain class 5 transcription factor 1 transcript variant 2 oct4b';POU domain class 5 transcription factor 1 transcript variant 3 oct4b;POU domain, class 5, transcription factor 1. |
| NCBI Gene Id | 18999 |
| Protein Name | POU domain, class 5, transcription factor 1 |
| Uniprot ID | P20263 |
| Protein Accession # | NP_038661.2 |
| Molecular Weight | 38.2; 25.0 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-OCT4 / POU5F1 (mouse) Antibody (OAEB00964)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Goat Anti-OCT4 / POU5F1 (mouse) Antibody (OAEB00964)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-OCT4 / POU5F1 (mouse) Antibody (OAEB00964)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-OCT4 / POU5F1 (mouse) Antibody (OAEB00964)"?
This target may also be called "NF-A3;Oct;Oct-;Oct3;Oct-3;Oct3/;Oct-3/;Oct3/4;Oct-3/4;Oct4;Oct-4;octamer-binding protein 3;octamer-binding protein 4;octamer-binding transcription factor 3;octamer-binding transcription factor 4 alternative;Otf;Otf-;Otf3;Otf3-;Otf-3;Otf3g;Otf3-rs7;Otf4;Otf-4;POU domain class 5 transcription factor 1 transcript variant 2 oct4b';POU domain class 5 transcription factor 1 transcript variant 3 oct4b;POU domain, class 5, transcription factor 1." in publications.
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What is the shipping cost for "Goat Anti-OCT4 / POU5F1 (mouse) Antibody (OAEB00964)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-OCT4 / POU5F1 (mouse) Antibody (OAEB00964)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-OCT4 / POU5F1 (mouse) Antibody (OAEB00964)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "38.2; 25.0".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-OCT4 / POU5F1 (mouse) Antibody (OAEB00964)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Pou5f1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Pou5f1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Pou5f1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Pou5f1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Pou5f1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Pou5f1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
