Goat Anti-NFATC2 / NFAT1 Antibody (OAEB02006)

Catalog No: OAEB02006 (Formerly GWB-EV0632)

Size:100UG

OAEB02006 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02006 (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: NFATC2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 86%; Rat: 93%
• Peptide Sequence: C-QQFRTGLSSPDARYQ
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:64000.
  Western Blot: Approx 110kDa band observed in Human lysates of cell line MOLT4 (calculated MW of 100kDa according to NP_775114.1). Recommended concentration: 0.3-1ug/ml.
• Reference: * Celil Aydemir AB, Lee S, Won Kim D, Gardner TR, Prince D, Mok Ahn J, Young-In Lee F,; Nuclear factor of activated T cell mediates proinflammatory gene expression in response to mechanotransduction.; Ann. N. Y. Acad. Sci. 2007 Nov 1117 : 138-42.; Pubmed 17584983

Target Info

• Gene Symbol: Igf2bp3|NFATC2
• Gene Full Name: insulin-like growth factor 2 mRNA binding protein 3|nuclear factor of activated T cells 2
• Alias Symbols: 2610101N11Rik;AA522010;Ab2-255;AL022933;AU045931;IGF2 mRNA-binding protein 3;IGF-II mRNA-binding protein 3;IM;IMP3;IMP-3;insulin-like growth factor 2 mRNA-binding protein 3;insulin-like growth factor 2, binding protein 3;Koc1;Koc13;mimp3;mIMP-3;Neilsen;NFAT pre-existing subunit;NFAT transcription complex, preexisting component;NFAT1;NF-ATc2;NFATP;nuclear factor of activated T-cells, cytoplasmic 2;nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2;nuclear factor of activated T-cells, preexisting component;preexisting nuclear factor of activated T-cells 2;RGD1306512;T cell transcription factor NFAT1;VICKZ family member 3.
• NCBI Gene Id: 140488|312320|4773
• Protein Name: Nuclear factor of activated T-cells, cytoplasmic 2
• Uniprot ID: Q13469
• Protein Accession #: NP_036472.2
• Molecular Weight: 99.8; 100; 97.7