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Datasheets/Manuals | Printable datasheet for Goat Anti-NEFM / NF-M Antibody (OAEB01149) |
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Predicted Species Reactivity | Bovine|Dog|Human|Mouse|Porcine|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | NEFM |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rat: 100% |
Peptide Sequence | C-QASHITVERKDYLK |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml. |
Reference | * Levy E, Liem RK, D'Eustachio P, Cowan NJ; Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein; European J. Biochem. 1987 Jul 166 (1): 71-7; Pubmed 3036526 |
Gene Symbol | Nefm|NEFM |
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Gene Full Name | neurofilament medium chain|neurofilament, medium polypeptide |
Alias Symbols | 160 kDa neurofilament protein;Nef;NEF3;neurofilament 3;neurofilament 3, medium;neurofilament medium polypeptide;neurofilament protein M;Neurofilament protein, middle polypeptide;neurofilament triplet M protein;neurofilament, medium polypeptide;neurofilament, medium polypeptide 150kDa;neurofilament-3 (150 kD medium);neurofilament-M;Nf;NF-;NF16;NF160;NF165;NFM;NF-M. |
NCBI Gene Id | 18040|24588|4741 |
Protein Name | Neurofilament medium polypeptide |
Uniprot ID | P07197 |
Protein Accession # | NP_005373.2 |
Molecular Weight | 102; 59.5 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-NEFM / NF-M Antibody (OAEB01149)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse|Porcine|Rat".
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How long will it take to receive "Goat Anti-NEFM / NF-M Antibody (OAEB01149)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-NEFM / NF-M Antibody (OAEB01149)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-NEFM / NF-M Antibody (OAEB01149)"?
This target may also be called "160 kDa neurofilament protein;Nef;NEF3;neurofilament 3;neurofilament 3, medium;neurofilament medium polypeptide;neurofilament protein M;Neurofilament protein, middle polypeptide;neurofilament triplet M protein;neurofilament, medium polypeptide;neurofilament, medium polypeptide 150kDa;neurofilament-3 (150 kD medium);neurofilament-M;Nf;NF-;NF16;NF160;NF165;NFM;NF-M." in publications.
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What is the shipping cost for "Goat Anti-NEFM / NF-M Antibody (OAEB01149)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-NEFM / NF-M Antibody (OAEB01149)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-NEFM / NF-M Antibody (OAEB01149)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "102; 59.5".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-NEFM / NF-M Antibody (OAEB01149)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Nefm|NEFM"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Nefm|NEFM"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Nefm|NEFM"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Nefm|NEFM"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Nefm|NEFM"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Nefm|NEFM"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.