Goat Anti-NCAM2 / OCAM Antibody (OAEB00572)

Catalog No: OAEB00572 (Formerly GWB-9EE351)

Size:100UG

OAEB00572 (0.5ug/ml) 24h-staining of PFA-perfused cryosection of Mouse Olfactory bub. Antigen retrieval with methanol (-20C, 10min) followed by 1% SDS(10min), IF-staning. Data obtained from anonymous customer.

OAEB00572 (0.5ug/ml) 48h-staining of PFA-perfused cryosection of Mouse Olfactory bulb. Antigen retrieval with citrate buffer pH 6 at 95C for 10min, IF-staning for NCAM2 (red), VGLUT2 (blue) and NQO1 (green), data obtained from anonymus customer.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: NCAM2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat, Dog, Cow
• Peptide Sequence: C-KVSNDIIQSKEDDSK
• Concentration: 0.5 mg/ml
• Application Info: P-ELISA: Antibody detection limit dilution 1:4000
  WB: reliminary experiments gave an approx 150 and 28kDa bands in Human and Mouse Brain lysates after 0.1 ug/ml antibody staining. Currently, an explanation cannot be found in the literature for the band observed given the calculated size of 93.0kDa according to NP_004531.2. Both observed bands were successfully blocked by incubation with the immunizing peptide.
  IHC: Frozen section of Olfactory bulb from transcardially PFA-perfused Mouse shows strong staining of axon bundles (right side middle, figure 1) and moderate peripheral staining outside the glomeruli (top, figure 1). NCAM2 (red) is stained complementary to VGLUT2 (blue) and NQO1 (green) in figure 2. Recommended concentration: 0.5 ug/ml. Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75 ug/ml.
• Reference: * Beecken WD, Engl T, Ogbomo H, Relja B, Cinatl J, Bereiter-Hahn J, Oppermann E, Jonas D, Blaheta RA.; Valproic acid modulates NCAM polysialylation and polysialyltransferase mRNA expression in human tumor cells.; Int Immunopharmacol. 2005 Apr;5(4):757-69.; Pubmed 15710344

Target Info

• Gene Symbol: Ncam2|NCAM2
• Gene Full Name: neural cell adhesion molecule 2
• Alias Symbols: fasciclin II;Ncam;NCAM-2;N-CAM-2;NCAM21;neural cell adhesion molecule 2;neural cell adhesion molecule RB-8;Oc;Ocam;OCAM-GPI;olfactory axon cell adhesion molecule;R4B12 antigen;RN;RNCAM.
• NCBI Gene Id: 17968|288280|4685
• Protein Name: Neural cell adhesion molecule 2
• Uniprot ID: O15394
• Protein Accession #: NP_004531.2
• Molecular Weight: 93