Goat Anti-MLLT10 / AF10 Antibody (OAEB01898)

Catalog No: OAEB01898 (Formerly GWB-EV0855)

Size:100UG

OAEB01898 (1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: MLLT10
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 92%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 92%; Zebrafish: 76%
• Peptide Sequence: C-RLEDTTARFTNAN
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Experiments gave bands at approx 140kDa and 60kDa in Rat Testis lysates. These bands correspond to earlier findings with different antibodies from other commercial sources.. This protein has a calculated MW of 109kDa according to Human NP_004632.1, 107kDa according to Human NP_001009569.1and 103kDa according to Rat NP_001012162.1. Recommended concentration: 1-3ug/ml.
• Reference: * Lin YH, Kakadia PM, Chen Y, Li YQ, Deshpande AJ, Buske C, Zhang KL, Zhang Y, Xu GL, Bohlander SK,; Global reduction of the epigenetic H3K79 methylation mark and increased chromosomal instability in CALM-AF10-positive leukemias.; Blood 2009 Jul 114 (3): 651-8.; Pubmed 19443658

Target Info

• Gene Symbol: Mllt10|MLLT10
• Gene Full Name: MLLT10 histone lysine methyltransferase DOT1L cofactor|MLLT10, histone lysine methyltransferase DOT1L cofactor|myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10
• Alias Symbols: Af1;AF10;ALL1-fused gene from chromosome 10 protein;B130021D15Rik;D630001B22Rik;mKIAA4140;myeloid/lymphoid or mixed lineage-leukemia translocation to 10 homolog;myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 10;myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10;protein AF-10;translocated to, 10;type I AF10 protein;type III AF10 protein;type IV AF10 protein.
• NCBI Gene Id: 17354|361285|8028
• Protein Name: Protein AF-10
• Uniprot ID: P55197
• Protein Accession #: NP_004632.1
• Molecular Weight: 109; 107