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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260) |
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| Predicted Species Reactivity | Human|Rat |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | WB |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | LIG1 |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 92%; Dog: 85%; Human: 100%; Mouse: 92%; Pig: 78%; Rat: 100% |
| Peptide Sequence | C-RVREDKQPEQATTS |
| Concentration | 0.5 mg/ml |
| Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Application Info | Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. |
| Reference | * Ferrari G, Rossi R, Arosio D, Vindigni A, Biamonti G, Montecucco A,; Cell cycle-dependent phosphorylation of human DNA ligase I at the cyclin-dependent kinase sites.; J. Biol. Chem. 2003 Sep 278 (39): 37761-7.; Pubmed 12851383 |
| Gene Symbol | Lig1|LIG1 |
|---|---|
| Gene Full Name | DNA ligase 1 |
| Alias Symbols | DNA ligase (ATP) 1;DNA ligase 1;DNA ligase I;ligase I, DNA, ATP-dependent;polydeoxyribonucleotide synthase [ATP] 1. |
| NCBI Gene Id | 3978|81513 |
| Protein Name | DNA ligase 1 |
| Uniprot ID | P18858 |
| Protein Accession # | NP_000225.1 |
| Molecular Weight | 102 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Rat".
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How long will it take to receive "Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260)"?
This target may also be called "DNA ligase (ATP) 1;DNA ligase 1;DNA ligase I;ligase I, DNA, ATP-dependent;polydeoxyribonucleotide synthase [ATP] 1." in publications.
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What is the shipping cost for "Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "102".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Lig1|LIG1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Lig1|LIG1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Lig1|LIG1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Lig1|LIG1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Lig1|LIG1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Lig1|LIG1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
