Goat Anti-GULP1 / CED6 Antibody (OAEB02459)

Catalog No: OAEB02459 (Formerly GWB-8C266B)

Size:100UG

OAEB02459 staining (0.2ug/ml) of Human Placenta lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

OAEB02459 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: GULP1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Horse: 92%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Zebrafish: 78%
• Peptide Sequence: EGTVFCLDPLDSRC
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:64000.
  Western Blot: Approx 35kDa band observed in Human Placenta lysates (calculated MW of 34.5kDa according to NP_057399.1). Recommended concentration: 0.2-0.6ug/ml.
  An additional band of unknown identity was also consistently observed at approx.46kDa. This band was successfully blocked by incubation with the immunising peptide.
• Reference: * Su HP, Nakada-Tsukui K, Tosello-Trampont AC, Li Y, Bu G, Henson PM,
  Ravichandran KS.; Interaction of CED-6/GULP, an adapter protein involved in engulfment of
  apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related
  protein (LRP).; J Biol Chem. 2002 Apr 5;277(14):11772-9. Epub 2001 Nov 29.; Pubmed 11729193

Target Info

• Gene Symbol: Gulp1|GULP1
• Gene Full Name: GULP PTB domain containing engulfment adaptor 1|GULP, engulfment adaptor PTB domain containing 1
• Alias Symbols: 3110030A04Rik;5730529O06Rik;CED 6;Ced6;CED-6;cell death protein 6 homolog;engulfment adapter protein;GU;GULP;GULP, engulfment adaptor PTB domain containing 1;Gulp-2;PTB domain adapter protein CED-6;PTB domain adaptor protein CED-6;PTB domain-containing engulfment adapter protein 1.
• NCBI Gene Id: 51454|70676
• Protein Name: PTB domain-containing engulfment adapter protein 1
• Uniprot ID: Q9UBP9
• Protein Accession #: NP_057399.1
• Molecular Weight: 34.5