Goat Anti-GRAP2 / GRID / Grf40 Antibody (OAEB01690)

Catalog No: OAEB01690 (Formerly GWB-D364CB)

Size:100UG

HEK293 overexpressing GRAP2 (RC206546) and probed with OAEB01690 (mock transfection in first lane), tested by Origene.

OAEB01690 staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: GRAP2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 92%; Human: 100%; Mouse: 92%; Rabbit: 92%; Rat: 92%
• Peptide Sequence: C-GLFPANYVAPMTR
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Approx 38kDa band observed lysates of cell line Jurkat (calculated MW of 37.9kDa according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.
• Reference: * Qiu M, Hua S, Agrawal M, Li G, Cai J, Chan E, Zhou H, Luo Y, Liu M.; Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1.; Biochem Biophys Res Commun. 1998 Dec 18;253(2):443-7.; Pubmed 9878555

Target Info

• Gene Symbol: Grap2|GRAP2
• Gene Full Name: GRB2 related adaptor protein 2|GRB2-related adaptor protein 2
• Alias Symbols: adapter protein GRID;G;Ga;GADS;GR;GRA;GRAP-2;GRB;GRB2L;GRB-2-like protein;GRB2-related adapter protein 2;Grb2-related adaptor downstream of Sch;GRB2-related adaptor protein 2;GRB-2-related monocytic adapter protein;GRB2-related protein with insert domain;GRBLG;GrbX;Grf4;grf-40;Grf40;grf40 adapter protein;GRID;growth factor receptor-binding protein;growth factor receptor-bound protein 2-related adaptor protein 2;GRPL;hematopoietic cell-associated adapter protein GrpL;hematopoietic cell-associated adaptor protein GRPL;M;Mona;monocytic adapter;monocytic adaptor;P3;P38;SH3-SH2-SH3 adapter Mona;SH3-SH2-SH3 adaptor molecule.
• NCBI Gene Id: 17444|9402
• Protein Name: GRB2-related adapter protein 2
• Uniprot ID: O75791
• Protein Accession #: NP_004801.1
• Molecular Weight: 37.9