- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for Goat Anti-FZD8 / frizzled 8 Antibody (OAEB02431) |
---|
Predicted Species Reactivity | Human, Mouse, Rat, Dog, Cow |
---|---|
Product Format | Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
Clonality | Polyclonal |
Host | Goat |
Application | WB, IHC |
Reconstitution and Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Immunogen | Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Peptide Sequence | C-SYPKQMPLSQV |
Concentration | 0.5 mg/ml |
Intended Use | Research use only. Not for diagnostic or therapeutic use. |
Application Info | P-ELISA: Antibody detection limit dilution 1:32000 WB: Approx 75kDa band observed in human heart and human pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3ug/ml. IHC: In paraffin embedded Human Pancreas, shows granular cytoplasmic staining of acinar cells. Cells of the Islets of Langerhans are weakly stained, if at all. Recommended concentration, 3ug/ml. IF: This antibody has been successfully used in IF on Mouse: Zhao B et al. (2019) PMID: 21321602. |
Reference | * Keay SK, Szekely Z, Conrads TP, Veenstra TD, Barchi JJ Jr, Zhang CO, Koch KR, Michejda CJ.; An antiproliferative factor from interstitial cystitis patients is a frizzled 8 protein-related sialoglycopeptide.; Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11803-8. Epub 2004 Jul 28.; Pubmed 15282374 |
Publications | Yin, S. et al. Tumor-initiating cells and FZD8 play a major role in drug resistance in triple-negative breast cancer. Mol. Cancer Ther. 12, 491-8 (2013). 23445611 |
Gene Symbol | Fzd8|FZD8 |
---|---|
Gene Full Name | frizzled class receptor 8 |
Alias Symbols | frizzled 8, seven transmembrane spanning receptor;frizzled family receptor 8;frizzled homolog 8;frizzled-8;fz-8;Fz8;hFZ8. |
NCBI Gene Id | 14370|8325 |
Protein Name | Frizzled-8 |
Uniprot ID | Q9H461 |
Protein Accession # | NP_114072.1 |
Molecular Weight | 73.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "Goat Anti-FZD8 / frizzled 8 Antibody (OAEB02431)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Dog, Cow".
-
How long will it take to receive "Goat Anti-FZD8 / frizzled 8 Antibody (OAEB02431)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
-
What buffer format is "Goat Anti-FZD8 / frizzled 8 Antibody (OAEB02431)" provided in?
This item is provided in "Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-FZD8 / frizzled 8 Antibody (OAEB02431)"?
This target may also be called "frizzled 8, seven transmembrane spanning receptor;frizzled family receptor 8;frizzled homolog 8;frizzled-8;fz-8;Fz8;hFZ8." in publications.
-
What is the shipping cost for "Goat Anti-FZD8 / frizzled 8 Antibody (OAEB02431)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "Goat Anti-FZD8 / frizzled 8 Antibody (OAEB02431)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "Goat Anti-FZD8 / frizzled 8 Antibody (OAEB02431)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "73.3".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-FZD8 / frizzled 8 Antibody (OAEB02431)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "Fzd8|FZD8"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "Fzd8|FZD8"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "Fzd8|FZD8"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "Fzd8|FZD8"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "Fzd8|FZD8"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "Fzd8|FZD8"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.