Goat Anti-EVC2 / Limbin Antibody (OAEB00126)

Catalog No: OAEB00126 (Formerly GWB-8549BB)

Size:100UG

OAEB00126 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: EVC2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Peptide Sequence: C-LNAKKAMRALGMD
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Consistent band observed in 293 and human heart at approx 75kDa with 1 hour incubation of primary at 0.5ug/ml.
• Reference: * Takeda H, Takami M, Oguni T, Tsuji T, Yoneda K, Sato H, Ihara N, Itoh T, Kata SR, Mishina Y, Womack JE, Moritomo Y, Sugimoto Y, Kunieda T.; Positional cloning of the gene LIMBIN responsible for bovine chondrodysplastic dwarfism.; Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10549-54.; Pubmed 12136126

Target Info

• Gene Symbol: EVC2
• Gene Full Name: EvC ciliary complex subunit 2
• Alias Symbols: Ellis van Creveld syndrome 2;ellis-van Creveld syndrome protein 2;LBN;limbin;WAD.
• NCBI Gene Id: 132884
• Protein Name: Limbin
• Uniprot ID: Q86UK5
• Protein Accession #: NP_667338.3
• Molecular Weight: 148; 140