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Datasheets/Manuals | Printable datasheet for Goat Anti-EIF2C1 / AGO1 Antibody (OAEB01475) |
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Predicted Species Reactivity | Dog|Human|Mouse|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB |
Additional Information | Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog |
:: | Description: This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4. |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | AGO1 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100% |
Peptide Sequence | C-KNASYNLDPYIQEF |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: In transfected HEK293 transiently expressing human EIF2C1bands of approx. 110kDa and 35kDa are observed. The 110kDa band is not observed in the non-transfected HEK293. The calculated molecular size is 97.2kDa according to NP_036331.1. Recommended concentration, 0.3-1ug/ml. |
Reference | * Gregory RI, Chendrimada TP, Cooch N, Shiekhattar R.; Human RISC couples microRNA biogenesis and posttranscriptional gene silencing.; Cell. 2005 Nov 18;123(4):631-40. Epub 2005 Nov 3.; Pubmed 16271387 |
Gene Symbol | AGO1 |
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Gene Full Name | argonaute RISC component 1 |
Alias Symbols | argonaute 1, RISC catalytic component;argonaute RISC catalytic component 1;argonaute1;EIF2C;eIF2C 1;eIF-2C 1;EIF2C1;eukaryotic translation initiation factor 2C, 1;GERP95;Golgi Endoplasmic Reticulum protein 95 kDa;hAgo1;protein argonaute-1;putative RNA-binding protein Q99;Q99. |
NCBI Gene Id | 26523 |
Protein Name | Protein argonaute-1 |
Uniprot ID | Q9UL18 |
Protein Accession # | NP_036331.1 |
Molecular Weight | 97.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-EIF2C1 / AGO1 Antibody (OAEB01475)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Dog|Human|Mouse|Rat".
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How long will it take to receive "Goat Anti-EIF2C1 / AGO1 Antibody (OAEB01475)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-EIF2C1 / AGO1 Antibody (OAEB01475)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-EIF2C1 / AGO1 Antibody (OAEB01475)"?
This target may also be called "argonaute 1, RISC catalytic component;argonaute RISC catalytic component 1;argonaute1;EIF2C;eIF2C 1;eIF-2C 1;EIF2C1;eukaryotic translation initiation factor 2C, 1;GERP95;Golgi Endoplasmic Reticulum protein 95 kDa;hAgo1;protein argonaute-1;putative RNA-binding protein Q99;Q99." in publications.
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What is the shipping cost for "Goat Anti-EIF2C1 / AGO1 Antibody (OAEB01475)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-EIF2C1 / AGO1 Antibody (OAEB01475)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-EIF2C1 / AGO1 Antibody (OAEB01475)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "97.2".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-EIF2C1 / AGO1 Antibody (OAEB01475)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "AGO1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "AGO1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "AGO1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "AGO1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "AGO1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "AGO1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.