Goat Anti-EDD1 / HYD Antibody (OAEB00347)

Catalog No: OAEB00347 (Formerly GWB-997462)

Size:100UG

OAEB00347 (4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: UBR5
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: C-LAIKTKNFGFV
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Approx. 35-40kDa band observed in Human Testis, Human Skeletal Muscle, A549 and A431 lysates at 0.5-1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted size of approx. 322kDa according to NP_056986 (furthermore, our SDS PAGE/western blotting protocol is not optimized for detection of proteins as large as the predicted). Currently we cannot find an explanation for this in the literature.
• Reference: * Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, Calanni ST, Jans DA, Sutherland RL, Watts CK.; EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response.; J Biol Chem. 2002 Jul 19;277(29):26468-78.; Pubmed 12011095

Target Info

• Gene Symbol: Ubr5|UBR5
• Gene Full Name: ubiquitin protein ligase E3 component n-recognin 5
• Alias Symbols: Dd5;E3 identified by differential display;E3 ubiquitin-protein ligase UBR5;E3 ubiquitin-protein ligase UBR5-like;E3 ubiquitin-protein ligase, HECT domain-containing 1;Edd;Edd1;extraembryonic development;HECT-type E3 ubiquitin transferase UBR5;HYD;hyperplastic discs protein homolog;progestin-induced protein;Rat100.
• NCBI Gene Id: 117060|51366|70790
• Protein Name: E3 ubiquitin-protein ligase UBR5
• Uniprot ID: O95071
• Protein Accession #: NP_056986.2
• Molecular Weight: 309