Goat Anti-DDX5 / p68 RNA helicase Antibody (OAEB02333)

Catalog No: OAEB02333 (Formerly GWB-18CDBF)

Size:100UG

OAEB02333 staining (0.1ug/ml) of A431 cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

OAEB02333 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data obtained using a previous batch.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: DDX5
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: C-PMIGYPMPTGYSQ
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:64000.
  Western Blot: Approx 70kDa band observed in human epidermoid carcinoma A431 lysates (calculated MW of 69.1kDa according to NP_004387). Recommended concentration: 0.11-0.3ug/ml.
  Other Notes: Immunofluorescence: A customer has reported successful staining at a dilution of 1:100 on HT-1080 cells with a Rhodamine conjugated secondary.
• Reference: * Fukuda T, Yamagata K, Fujiyama S, Matsumoto T, Koshida I, Yoshimura K, Mihara M, Naitou M, Endoh H, Nakamura T, Akimoto C, Yamamoto Y, Katagiri T, Foulds C, Takezawa S, Kitagawa H, Takeyama K, O'Malley BW, Kato S.; DEAD-box RNA helicase subunits of the Drosha complex are required for processing of rRNA and a subset of microRNAs.; Nat Cell Biol. 2007 May;9(5):604-11.; Pubmed 17435748
  * Iggo R, Gough A, Xu W, Lane DP, Spurr NK.; Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction.; Proc Natl Acad Sci U S A. 1989 Aug;86(16):6211-4.; Pubmed 2762324

Target Info

• Gene Symbol: Ddx5|DDX5
• Gene Full Name: DEAD box helicase 5|DEAD-box helicase 5
• Alias Symbols: 2600009A06Rik;ATP-dependent RNA helicase DDX5;DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 5;DEAD (Asp-Glu-Ala-Asp) box helicase 5;DEAD (Asp-Glu-Ala-Asp) box polypeptide 5;DEAD box protein 5;DEAD box RNA helicase DEAD1;DEAD box-5;DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5;DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD);G17P1;Hl;HLR1;HUMP68;mDEAD1;p6;p68;probable ATP-dependent RNA helicase DDX5;RNA helicase p68.
• NCBI Gene Id: 13207|1655
• Protein Name: Probable ATP-dependent RNA helicase DDX5
• Uniprot ID: P17844
• Protein Accession #: NP_004387.1
• Molecular Weight: 69.1