Goat Anti-CMG1 / CCDC2 / IFT74 Antibody (OAEB02484)

Catalog No: OAEB02484 (Formerly GWB-D34713)

Size:100UG

OAEB02484 staining (0.3ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

OAEB02484 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Figure 2: OAEB02484 (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor.)

Product Info

• Predicted Species Reactivity: Human|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC, IF
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: IFT74
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%; Rabbit: 100%
• Peptide Sequence: C-KTIVDALHSTSGN
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Approx 70-75kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2kDa according to AAK77221.1, but 51.4kDa according to NP_079379.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Momeni et al, BMC Neurol. 2006 Dec 13;6:44.; PMID: 17166276). Recommended concentration: 0.3-1ug/ml.
  Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in cytoplasm of hepatocytes. Recommended concentration, 5-10ug/ml.
  Other Notes: Immunofluorescence: It was successfully used at 2.5mg/ml to stain rat cortical neurons (see picture and PMID: 17166276).
• Other Applications Image 1 Data: A) OAEB02484 (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor.)
• Reference: * Momeni P, Schymick J, Jain S, Cookson MR, Cairns NJ, Greggio E, Greenway MJ, Berger S, Pickering-Brown S, Chio A, Fung HC, Holtzman DM, Huey ED, Wassermann EM, Adamson J, Hutton ML, Rogaeva E, St George-Hyslop P, Rothstein JD, Hardiman O, Grafman J, Singleton A, Hardy J, Traynor BJ.; Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD.; BMC Neurol. 2006 Dec 13;6:44; Pubmed 17166276
  * Bell SE, Mavila A, Salazar R, Bayless KJ, Kanagala S, Maxwell SA, Davis GE.; Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling.; J Cell Sci. 2001 Aug;114(Pt 15):2755-73.; Pubmed 11683410

Target Info

• Gene Symbol: Ift74|IFT74
• Gene Full Name: intraflagellar transport 74
• Alias Symbols: BBS22;capillary morphogenesis gene 1 protein;capillary morphogenesis protein 1;CCDC2;CMG1;CMG-1;coiled-coil domain containing 2;coiled-coil domain-containing protein 2;intraflagellar transport 74 homolog;intraflagellar transport protein 74 homolog.
• NCBI Gene Id: 313365|80173
• Protein Name: Intraflagellar transport protein 74 homolog
• Uniprot ID: Q96LB3
• Protein Accession #: NP_001092692.1
• Molecular Weight: 69.2