Goat Anti-CD32 / FCGR2B Antibody (OAEB01316)

Catalog No: OAEB01316 (Formerly GWB-633CA3)

Size:100UG

OAEB01316 (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing CD32 (RC211982) and probed with OAEB01316 (mock transfection in first lane), tested by Origene.

OAEB01316 (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC, P-ELISA
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: FCGR2B
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Peptide Sequence: C-PDALEEPDDQNRI
• Concentration: 0.5 mg/ml
• Specificity: This antibody is expected to recognise all four reported isoforms (NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_003992.3).
• Application Info: P-ELISA: antibody detection limit dilution 1:32000.
  WB: Approx 38kDa band observed in lysates of cell line Daudi (calculated MW of 34.0kDa according to NP_003992.3). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 ug/ml. Primary incubation was 1 hour.
  IHC:Paraffin embedded Human Placenta. Recommended concentration: 6-8 ug/ml.
• Reference: * McGaha TL, Sorrentino B, Ravetch JV.; Restoration of tolerance in lupus by targeted inhibitory receptor expression.; Science. 2005 Jan 28;307(5709):590-3.; Pubmed 15681388

Target Info

• Gene Symbol: FCGR2B
• Gene Full Name: Fc fragment of IgG receptor IIb
• Alias Symbols: CD32;CD32B;CDw32;Fc fragment of IgG, low affinity II, receptor for (CD32);Fc fragment of IgG, low affinity IIb, receptor (CD32);Fc fragment of IgG, low affinity IIb, receptor for (CD32);Fc gamma receptor IIb;Fc gamma RIIb;FCG2;fc-gamma RII-b;Fc-gamma RII-c;fc-gamma-RIIb;Fc-gamma-RIIc;FCGR2;FCGR2C;fcRII-b;FcRII-c;IGFR2;igG Fc receptor II-b;IgG Fc receptor II-c;low affinity immunoglobulin gamma Fc region receptor II-b;Low affinity immunoglobulin gamma Fc region receptor II-c.
• NCBI Gene Id: 2213
• Protein Name: Low affinity immunoglobulin gamma Fc region receptor II-b
• Uniprot ID: P31994
• Protein Accession #: NP_003992.3
• Molecular Weight: 34.0; 31.9; 31.9; 34.0; 33.4