Goat Anti-BAP / SIL1 Antibody (OAEB02245)

Catalog No: OAEB02245 (Formerly GWB-2E3390)

Size:100UG

OAEB02245 staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Reactivity Info: Tested: Human; Expected: Human
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: SIL1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 92%; Horse: 92%; Human: 100%; Mouse: 78%; Rabbit: 85%
• Peptide Sequence: C-ELLGSVNSLLKELR
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:8000.
  Western Blot: Approx 55-60 kDa band observed in Human Liver and Kidney extracts (predicted MW of 53kDa according to NP_071909.1). Recommended for use at 1-2ug/ml.
• Reference: * Liu Y, Bhat RA, Seestaller-Wehr LM, Fukayama S, Mangine A, Moran RA, Komm BS, Bodine PV, Billiard J.; The orphan receptor tyrosine kinase Ror2 promotes osteoblast differentiation and enhances ex vivo bone formation.; Mol Endocrinol. 2007 Feb;21(2):376-87.; Pubmed 17095577
  * Anttonen AK, Mahjneh I, Hamalainen RH, Lagier-Tourenne C, Kopra O, Waris L, Anttonen M, Joensuu T, Kalimo H, Paetau A, Tranebjaerg L, Chaigne D, Koenig M, Eeg-Olofsson O, Udd B, Somer M, Somer H, Lehesjoki AE.; The gene disrupted in Marinesco-Sjogren syndrome encodes SIL1, an HSPA5 cochaperone.; Nat Genet. 2005 Dec;37(12):1309-11.; Pubmed 16282978
  * Chung KT, Shen Y, Hendershot LM.; BAP, a Mammalian BiP-associated Protein, Is a Nucleotide Exchange Factor That Regulates the ATPase Activity of BiP.; J Biol Chem. 2002 Dec 6;277(49):47557-63.; Pubmed 12356756

Target Info

• Gene Symbol: SIL1
• Gene Full Name: SIL1 nucleotide exchange factor
• Alias Symbols: BAP;BiP-associated protein;MSS;nucleotide exchange factor SIL1;SIL1 homolog, endoplasmic reticulum chaperone;SIL1-like protein endoplasmic reticulum chaperone;ULG5.
• NCBI Gene Id: 64374
• Protein Name: Nucleotide exchange factor SIL1
• Uniprot ID: Q9H173
• Protein Accession #: NP_071909.1
• Molecular Weight: 52.6