Goat Anti-AIF1 / IBA1 (isoform 1 and 3) Antibody (OAEB01061)

Catalog No: OAEB01061 (Formerly GWB-F5FF96)

Size:100UG

AIF1 / IBA1 staining of Rat Brain lysate (35ug protein in RIPA buffer) by OAEB01061 (0.5ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.

(0.5ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

OAEB01061 (0.5ug/ml) staining of Mouse (A) and Rat (B Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

OAEB01061 (2ug/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

(4ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

OAEB01061 (6ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

OAEB01061 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-ELISA, WB, IHC
• Additional Information: Specific Reference: This antibody (previous batch) has been successfully used in IHC on Mouse:
  Recio JS, Álvarez-Dolado M, Díaz D, Baltanás FC, Piquer-Gil M, Alonso JR, Weruaga E.
  Bone marrow contributes simultaneously to different neural types in the central nervous system through different mechanisms of plasticity.
  Cell Transplant. 2011;20(8):1179-92.
  PMID: 21294954
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: AIF1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Peptide Sequence: C-TGPPAKKAISELP
• Concentration: 0.5 mg/ml
• Specificity: This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
• Application Info: P-ELISA: antibody detection limit dilution 1:128000.
  WB: Approx 16-17 kDa band observed in Human Lymph node and in Mouse and Rat Brain lysates (calculated MW of 16.7 kDa according to Human NP_001614.3 and 16.9 kDa according to Mouse NP_062340.1 and 16.8 kDa according to Rat NP_058892.1). Recommended concentration: 1-3 ug/ml. Primary incubation 1 hour at room temperature.
  IHC: Paraffin embedded Mouse Lung. Recommended concentration: 10 ug/ml.

Target Info

• Gene Symbol: Aif1|AIF1
• Gene Full Name: allograft inflammatory factor 1
• Alias Symbols: AI607846;AIF-1;allograft inflammatory factor 1;balloon angioplasty responsive transcript;balloon angioplasty-responsive transcript 1;Bart1;BART-1;D17H6S50;D17H6S50E;G1;Ib;Iba1;interferon gamma responsive transcript;ionized calcium binding adapter molecule 1;ionized calcium-binding adapter molecule 1;IRT1;IRT-1;microglia response factor;mrf-1;protein BART-1;protein G1;testis specific.
• NCBI Gene Id: 11629|199|29427
• Protein Name: Allograft inflammatory factor 1
• Uniprot ID: Q9UIV4
• Protein Accession #: NP_116573.1
• Molecular Weight: 10.5; 16.7