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Datasheets/Manuals | Printable datasheet for anti-GMNN antibody - OAPA00176 |
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Predicted Species Reactivity | Human |
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Product Format | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. |
Clonality | Monoclonal |
Clone | P2H7AT. |
Isotype | Mouse IgG1 heavy chain and kappa light chain. |
Host | Mouse |
Application | WB |
:: | Product Type: Mouse Anti Human Monoclonal. |
:: | Product Introduction: Geminin is a 25 kDa nuclear protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. In addition, Geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions. Geminin can also promote DNA replication. Geminin has 2 roles in 2 different stages of the cell cycle: Geminin is a negative regulator of DNA replication during the "S phase" of the cell cycle. Inhibition of Geminin during the "S phase" (by RNAi) results in an additional round of replication of portions of the genome. During the "M phase" of the cell cycle (mitosis) Geminin stabilizes the replication factor Cdt1 promoting DNA replication during the next cell cycle. Moreover, inhibition of Geminin during mitosis (by RNAi) causes destabilization of Cdt1 protein and impairment of DNA replication during the next cell cycle. G |
Reconstitution and Storage | For periods up to 1 month store at 4C, for longer periods of time, store at -20C. Prevent freeze thaw cycles. Stability: 12 months at -20C. 1 month at 4C. |
Immunogen | The immunogen for anti-GMNN antibody: anti-human Geminin mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Geminin amino acids 1-209 purified from E. coli. |
Purity | Geminin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. |
Application Info | Geminin antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GMNN Antibody (OAPA00176)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "GMNN Antibody (OAPA00176)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "GMNN Antibody (OAPA00176)" provided in?
This item is provided in "1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GMNN Antibody (OAPA00176)"?
This target may also be called "Gem, MGORS6" in publications.
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What is the shipping cost for "GMNN Antibody (OAPA00176)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GMNN Antibody (OAPA00176)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GMNN Antibody (OAPA00176)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GMNN Antibody (OAPA00176)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GMNN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GMNN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GMNN"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GMNN"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GMNN"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GMNN"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.