Catalog No: OKCA00463
Size:96 Wells
Price: $857.00
SKU
OKCA00463
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Glutamic Acid Decarboxylase Auto-Antibody (IgM) (Human) ELISA Kit (OKCA00463)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit PrincipleAviva Systems Biology Glutamic Acid Decarboxylase Auto-Antibody (IgM) (Human) ELISA Kit (OKCA00463) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Glutamic Acid Decarboxylase antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanIgM is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Glutamic Acid Decarboxylase Auto-Antibody (IgM) captured in well.
ELISA Kit ReproducibilityThree different samples of known Glutamic Acid Decarboxylase Auto-Antibody (IgM) concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
Intra-Assay CV: <15%
Inter-Assay CV: <15%
ELISA Kit Component
ComponentAmount
Glutamic Acid Decarboxylase Auto-Antibody (IgM) Microplate96 Wells (12 x 8 Well strips)
Standard 3 x 1 ml
Negative Control 1 x 1 ml
Positive Control 1 x 1 ml
Anti-Human IgM HRP-conjugate 1 x 10 ml
Sample Diluent 1 x 20 ml
25X Wash Buffer1 x 20 ml
Substrate A 1 x 5 ml
Substrate B 1 x 5 ml
Stop Solution 1 x 5 ml
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum
Assay InfoAssay Methodology: Qualitative Reverse Capture ELISA
Protocol Information
Alias SymbolsGAD
Protein NameGlutamic Acid Decarboxylase Auto-Antibody (IgM)
Description of TargetGlutamic Acid Decarboxylase Auto-Antibody (IgM)
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