Glutamic Acid Decarboxylase Antibody ELISA Kit (Human) (OKCD01298)

Catalog No: OKCD01298

Size:96WELLS

Human Glutamic Acid Decarboxylase ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h, 30min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-90%	82-90%	80-94%	95-102%
  EDTA Plasma (n=5)	98-105%	81-101%	84-99%	90-97%
  Heparin Plasma (n=5)	93-101%	78-95%	82-101%	85-94%

• ELISA Kit Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Glutamic Acid Decarboxylase Antibody (Anti-GAD) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Glutamic Acid Decarboxylase Antibody (Anti-GAD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	91-99	95
  EDTA Plasma (n=5)	79-93	85
  Heparin Plasma (n=5)	81-99	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Glutamic Acid Decarboxylase Antibody (Anti-GAD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Glutamic Acid Decarboxylase Antibody (Anti-GAD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Glutamic Acid Decarboxylase antibody Microplate: 96 Wells (12 x 8 Well strips)
  • Glutamic Acid Decarboxylase antibody Lyophilized Standard: 2 x XXX
  • 100X Detector Antibody: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.59 ng/mL
• Assay Info: Assay Method: Quantitative Reverse Capture Sandwich ELISA

Target Info

• Gene Symbol: GAD1
• Gene Full Name: glutamate decarboxylase 1
• Alias Symbols: 67 kDa glutamic acid decarboxylase;CPSQ1;DEE89;GAD;GAD-67;glutamate decarboxylase 1;glutamate decarboxylase 1 (brain, 67kDa);Glutamate decarboxylase 67 kDa isoform;SCP.
• NCBI Gene Id: 2571
• Protein Name: Glutamate decarboxylase 1
• Description of Target: Catalyzes the production of GABA.
• Uniprot ID: Q99259
• Nucleotide Accession #: NM_000818.2