GLUD1 ELISA Kit (Mouse) (OKCD00231)

Catalog No: OKCD00231 (Formerly GWB-KBBCA6)

Size:96WELLS

Mouse Glud1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	87-94%	83-90%	92-105%	92-105%
  EDTA plasma(n=5)	89-96%	81-103%	83-99%	93-101%
  heparin plasma(n=5)	94-102%	99-105%	90-102%	92-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Dehydrogenase 1 (GLUD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Dehydrogenase 1 (GLUD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Dehydrogenase 1 (GLUD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutamate Dehydrogenase 1 (GLUD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-93	80
  EDTA plasma(n=5)	79-99	95
  heparin plasma(n=5)	98-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Dehydrogenase 1 (GLUD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Dehydrogenase 1 (GLUD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Glud1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Glud1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Glud1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.065 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Dehydrogenase 1 (GDH). No significant cross-reactivity or interference between Glutamate Dehydrogenase 1 (GDH) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Glud1
• Gene Full Name: glutamate dehydrogenase 1
• Alias Symbols: AI118167;GDH 1;Gdh-X;Glu;Glud;Gludl;glutamate dehydrogenase 1, mitochondrial.
• NCBI Gene Id: 14661
• Protein Name: Glutamate dehydrogenase 1, mitochondrial
• Description of Target: Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
• Uniprot ID: P26443
• Protein Accession #: NP_032159
• Nucleotide Accession #: NM_008133.4
• Protein Size (# AA): 558