Catalog No: OKCA00420
Size:96 Wells
Price: $754.00
SKU
OKCA00420
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Gliadin Antibody (IgG) (Human) ELISA Kit (OKCA00420)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit PrincipleAviva Systems Biology Gliadin Antibody (IgG) (Human) ELISA Kit (OKCA00420) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Gliadin antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanIgG is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Gliadin Antibody (IgG) captured in well.
ELISA Kit ReproducibilityThree different samples of known Gliadin Antibody (IgG) concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
Intra-Assay CV: <15%
Inter-Assay CV: <15%
ELISA Kit Component
ComponentAmount
Gliadin Antibody (IgG) Microplate96 Wells (12 x 8 Well strips)
Negative Control1 x 800 uL
Positive Control1 x 800 uL
100X Anti-Human IgG HRP-Conjugate1 x 120 uL
HRP-Conjugate Diluent1 x 20 mL
Sample Diluent2 x 20 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum and Plasma
Assay InfoAssay Methodology: Qualitative Reverse Capture ELISA
Protein NameGliadin Antibody (IgG)
Description of TargetGliadin Antibody (IgG)
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