Catalog No: OKEH03884
Size:96T
Price: $625.00
SKU
OKEH03884
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03884
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GIP ELISA Kit (Pig) (OKEH03884) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-pig GIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GIP and incubated. The GIP found in the sample or standards competes with the biotinylated GIP for limited binding sites on the immobilized anti-pig GIP antibody. Excess unbound biotinylated GIP and sample or standard GIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GIP captured in the well and inversely proportional to the amount of GIP which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit Component
ComponentAmount
Anti-GIP Microplate96 Wells (12 x 8 Well strips)
GIP Lyophilized Standard2
Sample Diluent1 x 20 mL
100X GIP-Biotin Complex1 x 60 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity30 pg/mL
SpecificityNatural and recombinant Pig Gastric inhibitory polypeptide
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolGIP
Gene Full Namegastric inhibitory polypeptide
Alias SymbolsGlucose-dependent insulinotropic polypeptide.
NCBI Gene Id100533199
Protein NameP01281
Description of TargetGastric inhibitory polypeptide
Uniprot IDhttps://www.uniprot.org/uniprot/P01281
Protein Size (# AA)42
Molecular Weight5 kDa
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