Catalog No: OKEH03462
Size:96T
Price: $600.00
SKU
OKEH03462
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology GHRL ELISA Kit (Rat) (OKEH03462) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Ghrl antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ghrl and incubated. The Ghrl found in the sample or standards competes with the biotinylated Ghrl for limited binding sites on the immobilized anti-Rat Ghrl antibody. Excess unbound biotinylated Ghrl and sample or standard Ghrl is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ghrl captured in the well and inversely proportional to the amount of Ghrl which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.089 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Rat Appetite-regulating hormone | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Ghrl |
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Gene Full Name | ghrelin and obestatin prepropeptide |
Alias Symbols | appetite-regulating hormone;ghrelin/obestatin prepropeptide;growth hormone secretagogue;growth hormone-releasing peptide;motilin-related peptide. |
NCBI Gene Id | 59301 |
Protein Name | Q9QYH7 |
Description of Target | Appetite-regulating hormone |
Uniprot ID | https://www.uniprot.org/uniprot/Q9QYH7 |
Protein Accession # | NP_067701.1 |
Nucleotide Accession # | NM_021669.2 |
Protein Size (# AA) | 117 |
Molecular Weight | 13 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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