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Catalog No: OKEH03462
Size:96T
Price: $600.00
SKU
OKEH03462
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GHRL ELISA Kit (Rat) (OKEH03462) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Ghrl antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ghrl and incubated. The Ghrl found in the sample or standards competes with the biotinylated Ghrl for limited binding sites on the immobilized anti-Rat Ghrl antibody. Excess unbound biotinylated Ghrl and sample or standard Ghrl is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ghrl captured in the well and inversely proportional to the amount of Ghrl which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Component
ComponentAmount
Anti-Ghrl Microplate96 Wells (12 x 8 Well strips)
Ghrl Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Ghrl-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.089 ng/mL
SpecificityNatural and recombinant Rat Appetite-regulating hormone
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGhrl
Gene Full Nameghrelin and obestatin prepropeptide
Alias Symbolsappetite-regulating hormone;ghrelin/obestatin prepropeptide;growth hormone secretagogue;growth hormone-releasing peptide;motilin-related peptide.
NCBI Gene Id59301
Protein NameQ9QYH7
Description of TargetAppetite-regulating hormone
Uniprot IDhttps://www.uniprot.org/uniprot/Q9QYH7
Protein Accession #NP_067701.1
Nucleotide Accession #NM_021669.2
Protein Size (# AA)117
Molecular Weight13 kDa
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