GHRL ELISA Kit (Mouse) (OKEH03463)

Catalog No: OKEH03463

Size:96T

Mouse GHRL ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology GHRL ELISA Kit (Mouse) (OKEH03463) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Ghrl antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ghrl and incubated. The Ghrl found in the sample or standards competes with the biotinylated Ghrl for limited binding sites on the immobilized anti-Mouse Ghrl antibody. Excess unbound biotinylated Ghrl and sample or standard Ghrl is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ghrl captured in the well and inversely proportional to the amount of Ghrl which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.2% (n = 20)
  Mean Inter-assay CV%: <= 7.7% (n = 20)
• ELISA Kit Component:
  • Anti-Ghrl Microplate: 96 Wells (12 x 8 Well strips)
  • Ghrl Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Ghrl-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.164 ng/mL
• Specificity: Natural and recombinant Mouse Appetite-regulating hormone
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ghrl
• Gene Full Name: ghrelin
• Alias Symbols: 2210006E23Rik;appetite-regulating hormone;Gh;Ghr;growth hormone secretagogue;growth hormone-releasing peptide;m46;motilin-related peptide;MT;MTLRP;MTLRPAP;preproghrelin;proghrelin;Protein M46.
• NCBI Gene Id: 58991
• Protein Name: Q9EQX0
• Description of Target: Appetite-regulating hormone
• Uniprot ID: https://www.uniprot.org/uniprot/Q9EQX0
• Protein Accession #: NP_001273333.1
• Nucleotide Accession #: NM_001286404.1
• Protein Size (# AA): 117
• Molecular Weight: 13 kDa