GHR ELISA Kit (Mouse) (OKCD02568)

Catalog No: OKCD02568

Size:96WELLS

Mouse GHR ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-91%	93-105%	93-102%	78-90%
  EDTA Plasma (n=5)	80-98%	85-94%	81-99%	93-103%
  Heparin Plasma (n=5)	94-105%	79-97%	82-101%	78-89%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Hormone Receptor (GHR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Hormone Receptor (GHR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Hormone Receptor (GHR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Growth Hormone Receptor (GHR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-101	94
  EDTA Plasma (n=5)	79-96	86
  Heparin Plasma(n=5)	79-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone Receptor (GHR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone Receptor (GHR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GHR Microplate: 96 Wells (12 x 8 Well strips)
  • GHR Lyophilized Standard: 2
  • 100X Biotinylated GHR Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 34 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Hormone Receptor (GHR). No significant cross-reactivity or interference between Growth Hormone Receptor (GHR) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ghr
• Gene Full Name: growth hormone receptor
• Alias Symbols: GH receptor;GHB;GHBP;GHR/;GHR/BP;growth hormone receptor;Growth hormone receptor precursor (GH receptor) (GH binding protein) (GHBP) (Serum binding protein);growth hormone receptor/binding protein, high molecular weight;growth hormone receptor/binding protein, low molecular weight;somatotropin receptor.
• NCBI Gene Id: 14600
• Protein Name: Growth hormone receptor
• Description of Target: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to, and activates the JAK2/STAT5 pathway (By similarity).
• Uniprot ID: P16882
• Protein Accession #: NP_001041643.1
• Nucleotide Accession #: NM_001048178.2
• Protein Size (# AA): 650