Catalog No: OKEH00067
Size:96T
Price: $625.00
SKU
OKEH00067
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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GH1 ELISA Kit (Bovine) : 96 Wells (OKEH00067)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GH1 ELISA Kit (Bovine) (OKEH00067) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-bovine GH1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GH1 and incubated. The GH1 found in the sample or standards competes with the biotinylated GH1 for limited binding sites on the immobilized anti-bovine GH1 antibody. Excess unbound biotinylated GH1 and sample or standard GH1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GH1 captured in the well and inversely proportional to the amount of GH1 which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-GH1 Microplate96 Wells (12 x 8 Well strips)
GH1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X GH1-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.052 ng/mL
SpecificityNatural and recombinant Bovine Somatotropin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolGH1
Gene Full Namegrowth hormone 1
Alias SymbolsGH;Growth hormone;somatotropin.
NCBI Gene Id280804
Protein NameP01246
Description of TargetSomatotropin
Uniprot IDhttps://www.uniprot.org/uniprot/P01246
Protein Accession #NP_851339
Nucleotide Accession #NM_180996
Protein Size (# AA)217
Molecular Weight25 kDa
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