Catalog No: OKEH01667
Size:96T
Price: $525.00
SKU
OKEH01667
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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GFRA1 ELISA Kit (Human) : 96 Wells (OKEH01667)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GFRA1 ELISA Kit (Human) (OKEH01667) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GFRA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GFRA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GFRA1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 8.7% (n = 20)
ELISA Kit Component
ComponentAmount
GFRA1 Microplate96 Wells (12 x 8 Well strips)
GFRA1 Lyophilized Standard2
100X Biotinylated GFRA1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human GDNF family receptor alpha-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGFRA1
Gene Full NameGDNF family receptor alpha 1
Alias SymbolsGDNF family receptor alpha-1;GDNF receptor alpha 1d;GDNF receptor alpha 1e;GDNFR;GDNFRA;GDNFR-alpha-1;GFRalpha-1;GFR-ALPHA-1;Glial cell line-derived neurotrophic factor receptor alpha;GPI-linked anchor protein;PI-linked cell-surface accessory protein;RET ligand 1;RET1L;RETL1;TGF-beta-related neurotrophic factor receptor 1;TRNR1.
NCBI Gene Id2674
Protein NameP56159
Description of TargetGDNF family receptor alpha-1
Uniprot IDhttps://www.uniprot.org/uniprot/P56159
Protein Accession #NP_001138925.1
Nucleotide Accession #NM_001145453.2
Protein Size (# AA)465
Molecular Weight51 kDa
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