Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH06821
Size:96T
Price: $625.00
SKU
OKEH06821
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Gfm1 ELISA Kit (Mouse) (OKEH06821) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gfm1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gfm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gfm1 captured in the well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into serum = 82%
ELISA Kit ReproducibilityIntra-Assay Reproducibility CV% < 7.0% (n=20)< br>Inter-Assay Reproducibility CV% < 9.6% (n=24)
ELISA Kit Component
ComponentAmount
LIG1 Microplate96 Wells (12 x 8 Well strips)
LIG1 Lyophilized Standard2 x 5 ng
100X Biotinylated LIG1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity78 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGfm1
Gene Full NameG elongation factor, mitochondrial 1
Alias SymbolsAW545374;D3Wsu133;D3Wsu133e;EF-Gmt;elongation factor G 1, mitochondrial;elongation factor G, mitochondrial;elongation factor G1;G elongation factor 1;Gfm;mEF-G 1;mitochondrial.
NCBI Gene Id28030
Protein NameQ8K0D5
Description of TargetElongation factor G, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/Q8K0D5
Protein Accession #NP_613057
Nucleotide Accession #NM_138591
Write Your Own Review
You're reviewing:GFM1 ELISA Kit (Mouse) (OKEH06821)
Your Rating
We found other products you might like!