GFER ELISA Kit (Mouse) (OKCD01217)

Catalog No: OKCD01217 (Formerly GWB-KBBJM7)

Size:96WELLS

Mouse Gfer ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	78-99%	80-90%	80-89%	97-105%
  EDTA plasma(n=5)	92-99%	91-101%	90-102%	87-95%
  heparin plasma(n=5)	88-101%	84-103%	89-96%	80-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Factor, Augmenter Of Liver Regeneration (GFER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Factor, Augmenter Of Liver Regeneration (GFER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Factor, Augmenter Of Liver Regeneration (GFER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Growth Factor, Augmenter Of Liver Regeneration (GFER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	93-101	96
  EDTA plasma(n=5)	78-101	84
  heparin plasma(n=5)	97-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Factor, Augmenter Of Liver Regeneration (GFER) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Factor, Augmenter Of Liver Regeneration (GFER) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Gfer Microplate: 96 Wells (12 x 8 Well Strips)
  • Gfer Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated Gfer Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Factor, Augmenter Of Liver Regeneration (GFER). No significant cross-reactivity or interference between Growth Factor, Augmenter Of Liver Regeneration (GFER) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Gfer
• Gene Full Name: growth factor, augmenter of liver regeneration
• Alias Symbols: Alr;augmenter of liver regeneration;ER;ERV1;FAD-linked sulfhydryl oxidase ALR;growth factor, erv1-like (augmenter of liver regeneration).
• NCBI Gene Id: 11692
• Protein Name: FAD-linked sulfhydryl oxidase ALR
• Description of Target: FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen (By similarity).
• Uniprot ID: P56213
• Protein Accession #: NP_075527
• Nucleotide Accession #: NM_023040.3
• Protein Size (# AA): 198