Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH04596
Size:96T
Price: $525.00
SKU
OKEH04596
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Gdf15 ELISA Kit (Mouse) (OKEH04596) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gdf15 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Gdf15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gdf15 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Gdf15 Microplate96 Wells (12 x 8 Well Strips)
Gdf15 Lyophilized Standard2
Sample Diluent1 x 20 mL
Gdf15 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample Typeserum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
Sensitivity15.6 pg/mL
SpecificityNatural and recombinant Mouse Growth/differentiation factor 15
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGdf15
Gene Full Namegrowth differentiation factor 15
Alias Symbolsgrowth/differentiation factor 15;macrophage inhibiting cytokine-1;macrophage inhibitory cytokine 1;MIC-;MIC-1;NAG;NAG-1;SBF.
NCBI Gene Id23886
Protein NameQ9Z0J7
Description of TargetGrowth/differentiation factor 15
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Z0J7
Protein Accession #NP_035949.2
Nucleotide Accession #NM_011819.2
Protein Size (# AA)303
Molecular Weight33 KDa
Write Your Own Review
You're reviewing:Gdf15 ELISA Kit (Mouse) (OKEH04596)
Your Rating
We found other products you might like!