GDF15 Antibody (OALA06843)

Catalog No: OALA06843

Size:50 ug

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Mouse, Human
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: 6D12.H10.E4
• Isotype: IgG2a,l
• Host: Rat
• Conjugation: Unconjugated
• Application: ELISA, IHC, IHC-P, WB
• Reconstitution and Storage: Short term: store at 4C. Long term: aliquot and store at -20C. Avoid freeze-thaw cycles. Store undiluted.
• Immunogen: This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to C-term mouse NAG-1 protein.  Cross reactivity to MBP is negative.
• Purification: Protein G affinity chromatography
• Concentration: 1 mg/ml
• Specificity: This antibody reacts with the C-terminus of endogenous NAG-1 protein from mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
• Warning: For research use only.
• Application Info: ELISA (1:2000), IHC, IHC-P (10 ug/ml), WB (1:1000)
• Application Data: This Protein G purified antibody is suitable for ELISA and western blotting of human NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately 13 and 26 kD in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.
• Storage Buffer: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
• Protein Range: C-Terminus

Target Info

• Gene Symbol: GDF15
• Alias Symbols: GDF15, MIC1, MIC-1, NAG-1, NRG-1, NSAID-regulated gene 1 protein, PLAB, NSAID-activated gene 1 protein, Placental TGF-beta, PTGFB, GDF-15, PTGF-beta
• NCBI Gene Id: 9518
• Uniprot ID: Q99988
• Protein Accession #: NP_004855.2
• Nucleotide Accession #: NM_004864