GDF-15 ELISA Kit (Mouse) (OKBB00507)

Catalog No: OKBB00507

Size:96 Wells

Mouse GDF-15 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from sheep specific for GDF-15 has been precoated onto 96-well plates. Standards(NS0, S189-A303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse GDF-15 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	114	346	548	98.2	359	579
  SD	4.33	12.46	22.47	7.37	20.1	39.37
  CV%	3.8	3.6	4.1	7.5	5.6	6.8

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse GDF-15 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse GDF-15 Standard: 2 x 1 ng/tube
  • 100X Biotinylated Anti-Mouse GDF-15 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse GDF-15
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S189-A303
• Description: For quantitative detection of mouse GDF-15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: GDF15
• Gene Full Name: growth differentiation factor 15
• Alias Symbols: gdf 15, GDF15, GDF-15, MIC 1, MIC1, MIC-1, NAG-1, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, PDF, PLAB, PTGFB
• NCBI Gene Id: 23886
• Protein Name: Growth/differentiation factor 15
• Description of Target: GDF-15 (Growth differentiation factor 15), also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.
• Uniprot ID: Q9Z0J7
• Protein Accession #: NP_035949.2
• Nucleotide Accession #: NM_011819.2