GCDH antibody - C-terminal region (ARP43559_T100)

Catalog#: ARP43559_T100

• Size: 100ug

Human HepG2

Human Muscle

• Description of Target: GCDH belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits.The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Gene Symbol: GCDH
• Official Gene Full Name: Glutaryl-CoA dehydrogenase
• Alias Symbols: ACAD5; GCD
• Protein Accession#: NP_000150
• Nucleotide Accession#: NM_000159
• Swissprot Id: Q92947
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 438
• Molecular Weight: 48kDa
• Application: WB, IHC
• Partner Proteins: CSTF2, EEF1G, PAFAH1B3, SERPINB9, TIMP1, TLR10, CSTF2, EEF1G, PAFAH1B3, SERPINB9, TIMP1
• Immunogen: The immunogen for anti-GCDH antibody: synthetic peptide directed towards the C terminal of human GCDH
• Product Format: Lyophilized powder
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Bovine: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%; African clawed frog: 92%
• Peptide Sequence: Synthetic peptide located within the following region: IARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITG
• Blocking Peptide: For anti-GCDH antibody is Catalog # AAP43559 (Previous Catalog # AAPP25004)
• Additional Information: IHC Information: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.
• Key Reference: Kimura,K., (2006) Genome Res. 16 (1), 55-65
• Reconstitution and Storage: Add 100 μl of distilled water. Final anti-GCDH antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: GCDH antibody tested by IHC with human muscle (ARP43559)

Aviva Systems Biology is the original manufacturer of this GCDH antibody.

Click here to view the GCDH antibody Immunohistochemistry (IHC) protocol

Product Datasheet Link: GCDH antibody (ARP43559)

IHC Information:

Rabbit Anti-GCDH Antibody
Catalog Number: ARP43559
Paraffin Embedded Tissue: Human Muscle
Cellular Data: Skeletal muscle cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

IHC Image: