GBA Antibody - middle region (OAAB05533)

Catalog No: OAAB05533

Size:200UL

Western blot analysis of GC Antibody (Center) (Cat. #OAAB05533) in mouse lung tissue lysates (35ug/lane). GC (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human Skin reacted with GC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

GC Antibody (Center) (Cat. #OAAB05533) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Bovine, Human, Mouse, Porcine
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Flow cytometry, Immunohistochemistry-Paraffin, Western blot
  Cellular Location: Lysosome membrane; Peripheral membrane protein; Lumenal side. Note=Interaction with saposin-C promotes membrane association. Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This GC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337-365 amino acids of human GC.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Bovine, Pig
• Concentration: Varies by lot. See vial for concentration.
• Intended Use: GC Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: FC: 1:25
  WB: 1:1000
  IHC-P-Leica: 1:500
• Other Applications Image 1 Data: GC Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: GBA
• Gene Full Name: glucosylceramidase beta
• Alias Symbols: acid beta-glucosidase;alglucerase;beta-GC;beta-glucocerebrosidase;cholesterol glucosyltransferase;cholesteryl-beta-glucosidase;D-glucosyl-N-acylsphingosine glucohydrolase;GBA1;GCB;GLUC;glucocerebrosidase;glucosidase, beta, acid;glucosylceramidase-like protein;imiglucerase;lysosomal acid GCase;lysosomal acid glucosylceramidase;lysosomal glucocerebrosidase;SGTase.
• NCBI Gene Id: 2629
• Protein Name: NP_000148.2, NP_001005741.1, NP_001005742.1, P04062, Q2KHZ8, Q70KH2
• Description of Target: Glucosylceramidase, glucosylceramidase isoform 1 precursor [Homo sapiens]
• Uniprot ID: https://www.uniprot.org/uniprot/?query=P04062+OR+Q2KHZ8+OR+Q70KH2
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_000148.2,NP_001005741.1,NP_001005742.1
• Molecular Weight: 59716