GATA2 Antibody - N-terminal region : HRP (ARP31855_T100-HRP)

Catalog No: ARP31855_T100-HRP

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: IHC, WB
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GATA2
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: PYYANPAHARARVSYSPAHARLTGGQMCRPHLLHSPGLPWLDGGKAALSA
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-GATA2 (ARP31855_T100-HRP) antibody is Catalog # AAP31855 (Previous Catalog # AAPP02650)
• Sample Type Confirmation:

  GATA2 is strongly supported by BioGPS gene expression data to be expressed in K562

• Reference: Tsuzuki, S., et al., (2004) Mol. Cell. Biol. 24 (15), 6824-6836
• Publications:

  Mammoto, A. et al. A mechanosensitive transcriptional mechanism that controls angiogenesis. Nature 457, 1103-8 (2009). WB, IHC, IP, EMSA, ChIP, Rat, Human, Bovine, Dog, Horse, Rabbit, Guinea pig, Mouse 19242469

  Huang, Y.-J. et al. The functional IGFBP7 promoter -418G>A polymorphism and risk of head and neck cancer. Mutat. Res. 702, 32-9 (2010). WB, IHC, IP, EMSA, ChIP, Rat, Human, Bovine, Dog, Horse, Rabbit, Guinea pig, Mouse 20599521

  Jack, B. H. A. & Crossley, M. GATA proteins work together with friend of GATA (FOG) and C-terminal binding protein (CTBP) co-regulators to control adipogenesis. J. Biol. Chem. 285, 32405-14 (2010). WB, IHC, IP, EMSA, ChIP, Rat, Human, Bovine, Dog, Horse, Rabbit, Guinea pig, Mouse 20705609

  Fujiwara, T. et al. Gene expression profiling identifies HOXB4 as a direct downstream target of GATA-2 in human CD34+ hematopoietic cells. PLoS One 7, e40959 (2012). WB, IHC, IP, EMSA, ChIP, Rat, Human, Bovine, Dog, Horse, Rabbit, Guinea pig, Mouse 23028422

  Wang, F. et al. A regulatory circuit comprising GATA1/2 switch and microRNA-27a/24 promotes erythropoiesis. Nucleic Acids Res. 42, 442-57 (2014). WB, IHC, IP, EMSA, ChIP, Rat, Human, Bovine, Dog, Horse, Rabbit, Guinea pig, Mouse 24049083

Target Info

• Gene Symbol: GATA2
• Gene Full Name: GATA binding protein 2
• Alias Symbols: DCML, IMD21, NFE1B, MONOMAC
• NCBI Gene Id: 2624
• Protein Name: Endothelial transcription factor GATA-2
• Description of Target: The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells is essential for normal primitive and definitive erythropoiesis and is expressed at high levels in erythroid cells, mast cells, and megakaryocytes. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.
• Uniprot ID: P23769
• Protein Accession #: NP_116027
• Nucleotide Accession #: NM_032638
• Protein Size (# AA): 480
• Molecular Weight: 50kDa
• Protein Interactions: NOTCH2NL; KRTAP10-3; KRTAP10-9; KRT40; PRR20A; ADAMTSL4; TRAF1; PSMA3; MDFI; GOLGA2; FHL3; TRIM23; AKT1; BMI1; SPI1; SMAD4; ELAVL1; KAT2A; EP300; MED1; POU1F1; POU2AF1; ZBTB32; ZFPM1; ZBTB16; HDAC5; HDAC3; HHEX; RARA; RXRA; MAPK1; JUN; CEBPA; STAT3; PML;