Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OAPA00361 (Formerly GWB-BSP836)
Size:5UG
Price: $75.00
SKU
OAPA00361
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for anti-GAPDH antibody - OAPA00361
Product Info
Predicted Species ReactivityHuman
Product Format1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
ClonalityMonoclonal
ClonePAT8G4AT
IsotypeMouse IgG2b heavy chain and kappa light chain.
HostMouse
ApplicationIF, WB
::Product Introduction: GAPDH is a catalytic enzyme normally known to play a role in glycolysis. GAPDH exists as a tetramer composed of 36-kDa subunits and has a range of intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, GAPDH has function in intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. GAPDH catalyzes a vital energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Reconstitution and StorageFor periods up to 1 month store at 4C, for longer periods of time, store at -20C. Prevent freeze thaw cycles.
Stability: 12 months at -20C. 1 month at 4C.
ImmunogenThe immunogen for anti-GAPDH antibody: anti-human GAPDH mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GAPDH amino acids 1-335 purified from E. coli.
Predicted Homology Based on Immunogen SequenceHuman
PurityGAPDH antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Application InfoGAPDH antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 10,000. Recommended starting dilution is 1:1,000.
Publications

Lysosome purinergic receptor P2X4 regulates neoangiogenesis induced by microvesicles from sarcoma patients. Cell Death Dis. 12, 797 (2021)

Description
Gene SymbolGAPDH
Alias SymbolsG3PD, GAPD, HEL-S-162eP
NCBI Gene Id2597
Protein NameGlyceraldehyde-3-phosphate dehydrogenase
Description of TargetMouse Anti Human Glyceraldehyde-3-Phosphate Dehydrogenase
Uniprot IDP04406
Protein Accession #NP_001243728.1
  1. What is the species homology for "GAPDH Antibody (OAPA00361)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".

  2. How long will it take to receive "GAPDH Antibody (OAPA00361)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "GAPDH Antibody (OAPA00361)" provided in?

    This item is provided in "1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "GAPDH Antibody (OAPA00361)"?

    This target may also be called "G3PD, GAPD, HEL-S-162eP" in publications.

  5. What is the shipping cost for "GAPDH Antibody (OAPA00361)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "GAPDH Antibody (OAPA00361)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "GAPDH Antibody (OAPA00361)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "GAPDH Antibody (OAPA00361)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "GAPDH"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "GAPDH"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "GAPDH"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "GAPDH"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "GAPDH"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "GAPDH"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

Write Your Own Review
You're reviewing:GAPDH Antibody (OAPA00361)
Your Rating
We found other products you might like!