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GAPDH Antibody (OAPA00361)
Datasheets/Manuals | Printable datasheet for anti-GAPDH antibody - OAPA00361 |
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Predicted Species Reactivity | Human |
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Product Format | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. |
Clonality | Monoclonal |
Clone | PAT8G4AT |
Isotype | Mouse IgG2b heavy chain and kappa light chain. |
Host | Mouse |
Application | IF, WB |
:: | Product Introduction: GAPDH is a catalytic enzyme normally known to play a role in glycolysis. GAPDH exists as a tetramer composed of 36-kDa subunits and has a range of intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, GAPDH has function in intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. GAPDH catalyzes a vital energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. |
Reconstitution and Storage | For periods up to 1 month store at 4C, for longer periods of time, store at -20C. Prevent freeze thaw cycles. Stability: 12 months at -20C. 1 month at 4C. |
Immunogen | The immunogen for anti-GAPDH antibody: anti-human GAPDH mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GAPDH amino acids 1-335 purified from E. coli. |
Predicted Homology Based on Immunogen Sequence | Human |
Purity | GAPDH antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. |
Application Info | GAPDH antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 10,000. Recommended starting dilution is 1:1,000. |
Publications | Lysosome purinergic receptor P2X4 regulates neoangiogenesis induced by microvesicles from sarcoma patients. Cell Death Dis. 12, 797 (2021) |
Description |
Gene Symbol | GAPDH |
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Alias Symbols | G3PD, GAPD, HEL-S-162eP |
NCBI Gene Id | 2597 |
Protein Name | Glyceraldehyde-3-phosphate dehydrogenase |
Description of Target | Mouse Anti Human Glyceraldehyde-3-Phosphate Dehydrogenase |
Uniprot ID | P04406 |
Protein Accession # | NP_001243728.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GAPDH Antibody (OAPA00361)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "GAPDH Antibody (OAPA00361)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "GAPDH Antibody (OAPA00361)" provided in?
This item is provided in "1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GAPDH Antibody (OAPA00361)"?
This target may also be called "G3PD, GAPD, HEL-S-162eP" in publications.
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What is the shipping cost for "GAPDH Antibody (OAPA00361)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GAPDH Antibody (OAPA00361)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GAPDH Antibody (OAPA00361)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GAPDH Antibody (OAPA00361)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GAPDH"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GAPDH"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GAPDH"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GAPDH"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GAPDH"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GAPDH"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.