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Datasheets/Manuals | Printable datasheet for GAPDH Antibody - C-terminal region (OAEB02389) |
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Predicted Species Reactivity | Dog|Human|Mouse|Porcine|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB |
Additional Information | Reactivity Info: Tested: Human, Mouse; Expected: Human, Mouse, Dog |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | GAPDH |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Horse: 90%; Human: 100%; Pig: 90%; Sheep: 90% |
Peptide Sequence | C-HQVVSSDFNSDT |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:2000. Western Blot: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037). Recommended concentration: 0.01-0.03ug/ml. |
Reference | * Carr AJ, Vugler A, Lawrence J, Chen LL, Ahmado A, Chen FK, Semo M, Gias C, da Cruz L, Moore HD, Walsh J, Coffey PJ.; Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay.; Mol Vis. 2009;15:283-95. Epub 2009 Feb 6.; Pubmed 19204785 * Kiepe D, Van Der Pas A, Ciarmatori S, Standker L, Schutt B, Hoeflich A, Hugel U, Oh J, Tonshoff B.; Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein 2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I.; Endocrinology. 2008 Oct;149(10):4901-11. Epub 2008 Jun 12.; Pubmed 18556354 * Henson MA, Roberts AC, Salimi K, Vadlamudi S, Hamer RM, Gilmore JH, Jarskog LF, Philpot BD.; Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex.; Cereb Cortex. 2008 Nov;18(11):2560-73.; Pubmed 18296432 * Gerstner JR, Bremer QZ, Vander Heyden WM, Lavaute TM, Yin JC, Landry CF.; Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain.; PLoS One. 2008 Feb 20;3(2):e1631.; Pubmed 18286188 * Nguyen M, Pace AJ, Koller BH.; Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.; J Exp Med. 2007 Jun 11;204(6):1383-93.; Pubmed 17517966 * Burke JR, Enghild JJ, Martin ME, Jou YS, Myers RM, Roses AD, Vance JM, Strittmatter WJ.; Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH.; Nat Med. 1996 Mar;2(3):347-50.; Pubmed 8612237 |
Gene Symbol | GAPDH |
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Gene Full Name | glyceraldehyde-3-phosphate dehydrogenase |
Alias Symbols | aging-associated gene 9 protein;epididymis secretory sperm binding protein Li 162eP;G3PD;GAPD;glyceraldehyde-3-phosphate dehydrogenase;HEL-S-162eP;OCAS, p38 component;Oct1 coactivator in S phase, 38 Kd component;peptidyl-cysteine S-nitrosylase GAPDH. |
NCBI Gene Id | 2597 |
Protein Name | Glyceraldehyde-3-phosphate dehydrogenase |
Uniprot ID | P04406 |
Protein Accession # | NP_002037.2 |
Molecular Weight | 36.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GAPDH Antibody - C-terminal region (OAEB02389)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Dog|Human|Mouse|Porcine|Rat".
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How long will it take to receive "GAPDH Antibody - C-terminal region (OAEB02389)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "GAPDH Antibody - C-terminal region (OAEB02389)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GAPDH Antibody - C-terminal region (OAEB02389)"?
This target may also be called "aging-associated gene 9 protein;epididymis secretory sperm binding protein Li 162eP;G3PD;GAPD;glyceraldehyde-3-phosphate dehydrogenase;HEL-S-162eP;OCAS, p38 component;Oct1 coactivator in S phase, 38 Kd component;peptidyl-cysteine S-nitrosylase GAPDH." in publications.
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What is the shipping cost for "GAPDH Antibody - C-terminal region (OAEB02389)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GAPDH Antibody - C-terminal region (OAEB02389)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GAPDH Antibody - C-terminal region (OAEB02389)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "36.1".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GAPDH Antibody - C-terminal region (OAEB02389)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GAPDH"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GAPDH"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GAPDH"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GAPDH"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GAPDH"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GAPDH"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.