GALNT7 Antibody (OAGA00457)

Catalog No: OAGA00457

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
OAGA00457 diluted at 1:10000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
OAGA00457 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded Endometrial CA, using GALNT7(OAGA00457) antibody at 1:100 dilution.

GALNT7 antibody [N1C2] detects GALNT7 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GALNT7 protein stained by GALNT7 antibody [N1C2] (OAGA00457) diluted at 1:500.
Blue: Hoechst 33343 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: This gene encodes GalNAc transferase 7, a member of the GalNAc-transferase family. The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. This protein may function as a follow-up enzyme in the initiation step of O-glycosylation. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain
  Cellular Localization: Golgi apparatus membrane; Single-pass type II membrane protein
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 24 and 515 of GALNT7 (Uniprot ID#Q86SF2)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: GALNT7
• Gene Full Name: N-acetylgalactosaminyltransferase 7
• Alias Symbols: GalNAcT7, GALNAC-T7
• NCBI Gene Id: 51809
• Protein Name: N-acetylgalactosaminyltransferase 7
• Uniprot ID: Q86SF2
• Protein Accession #: NP_059119.2
• Molecular Weight: 75 kDa