GADD45G ELISA Kit (Human) (OKEH01665)

Catalog No: OKEH01665

Size:96T

Human GADD45G ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology GADD45G ELISA Kit (Human) (OKEH01665) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GADD45G has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GADD45G is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GADD45G captured in well.
• ELISA Kit Range: 78-5000pg/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.0% (n = 20)
  Mean Inter-assay CV%: < 7.9% (n = 20)
• ELISA Kit Component:
  • GADD45G Microplate: 96 Wells (12 x 8 Well strips)
  • GADD45G Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated GADD45G Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 39.2 pg/mL
• Specificity: Natural and recombinant Human Growth arrest and DNA damage-inducible protein GADD45 gamma
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GADD45G
• Gene Full Name: growth arrest and DNA damage inducible gamma
• Alias Symbols: CR6;cytokine-responsive protein CR6;DDIT2;DDIT-2;DNA damage-inducible transcript 2 protein;GADD45gamma;GADD45-gamma;gadd-related protein, 17 kD;growth arrest and DNA damage-inducible protein GADD45 gamma;GRP17.
• NCBI Gene Id: 10912
• Protein Name: O95257
• Description of Target: Growth arrest and DNA damage-inducible protein GADD45 gamma
• Uniprot ID: https://www.uniprot.org/uniprot/O95257
• Protein Accession #: NP_006696.1
• Nucleotide Accession #: NM_006705.3
• Protein Size (# AA): 159
• Molecular Weight: 17 kDa