GAD2 Antibody (OAGA00033)

Catalog No: OAGA00033

Size:100 ul

Sample (20 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
OAGA00033 diluted at 1:10000

Sample (10 ug of whole cell lysate)
A: rat brain
7.5% SDS PAGE
OAGA00033 diluted at 1:10000

GAD65 antibody [C2C3], C-term detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
GAD65 antibody [C2C3], C-term (OAGA00033) dilution: 1:500.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq]
  Positive Controls: mouse brain, rat brain
  Cellular Localization: Cytoplasm, cytosol, Cytoplasmic vesicle, Cell junction, synapse, presynaptic cell membrane, Golgi apparatus membrane
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 465 and 558 of GAD65 (Uniprot ID#Q05329)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: GAD2
• Gene Full Name: Glutamate decarboxylase 2
• Alias Symbols: GAD65
• NCBI Gene Id: 2572
• Protein Name: Glutamate decarboxylase 2
• Uniprot ID: Q05329
• Protein Accession #: NP_000809.1
• Molecular Weight: 65 kDa