Catalog No: OKEH00905
Size:96T
Price: $525.00
SKU
OKEH00905
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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GAD1 ELISA Kit (Rat) : 96 Wells (OKEH00905)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GAD1 ELISA Kit (Rat) (OKEH00905) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GAD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GAD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GAD1 captured in well.
ELISA Kit Range0.39-25ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
GAD1 Microplate96 Wells (12 x 8 Well strips)
GAD1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated GAD1 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.198 ng/mL
SpecificityNatural and recombinant Rat Glutamate decarboxylase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGad1
Gene Full Nameglutamate decarboxylase 1
Alias Symbols67 kDa glutamic acid decarboxylase;Gad67;GAD-67;glutamate decarboxylase 1;Glutamate decarboxylase 1 (brain);glutamate decarboxylase 1 variant GAD67NT;Glutamate decarboxylase 67 kDa isoform;glutamic acid decarboxylase 1.
NCBI Gene Id24379
Protein NameP18088
Description of TargetGlutamate decarboxylase 1
Uniprot IDhttps://www.uniprot.org/uniprot/P18088
Protein Accession #NP_058703.1
Nucleotide Accession #NM_017007.1
Protein Size (# AA)593
Molecular Weight66 kDa
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