Catalog No: OKEH04148
Size:96T
Price: $525.00
SKU
OKEH04148
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Gaa ELISA Kit (Mouse) (OKEH04148) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gaa has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gaa is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gaa captured in the well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.0% (n = 20)
Mean Inter-assay CV%: <= 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Gaa Microplate96 Wells (12 x 8 Well Strips)
Gaa Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Gaa Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.4 ng/mL
SpecificityNatural and recombinant Mouse Lysosomal alpha-glucosidase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGaa
Gene Full Nameglucosidase, alpha, acid
Alias Symbolsacid maltase;E430018M07Rik;lysosomal alpha-glucosidase.
NCBI Gene Id14387
Protein NameP70699
Description of TargetLysosomal alpha-glucosidase
Uniprot IDhttps://www.uniprot.org/uniprot/P70699
Protein Accession #NP_001152796.1
Nucleotide Accession #NM_001159324.2
Protein Size (# AA)953
Molecular Weight106 kDa
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