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Catalog No: OKEH00271 (Formerly GWB-8F69KO)
Size:96T
Price: $525.00
SKU
OKEH00271
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00271
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GAA ELISA Kit (Human) (OKEH00271) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GAA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for GAA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GAA captured in well.
ELISA Kit Range3.12-200ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
ELISA Kit Component
ComponentAmount
GAA Microplate96 Wells (12 x 8 Well strips)
GAA Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated GAA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.98 ng/mL
SpecificityNatural and recombinant Human Lysosomal alpha-glucosidase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGAA
Gene Full Namealpha glucosidase
Alias Symbolsacid maltase;aglucosidase alfa;glucosidase alpha, acid;LYAG;lysosomal alpha-glucosidase.
NCBI Gene Id2548
Protein NameP10253
Description of TargetLysosomal alpha-glucosidase
Uniprot IDhttps://www.uniprot.org/uniprot/P10253
Protein Accession #NP_000143.2
Nucleotide Accession #NM_000152.4
Protein Size (# AA)952
Molecular Weight105 kDa
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