Catalog No: OKEH04628
Size:96T
Price: $525.00
SKU
OKEH04628
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology GA ELISA Kit (Human) (OKEH04628) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for GA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GA captured in well.
ELISA Kit Range15.6-1000ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-GA Microplate96 Wells (12 x 8 Well Strips)
GA Lyophilized Standard2
Sample Diluent1 x 20 mL
GA Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity7.495 ng/mL
SpecificityNatural and recombinant Human Glycated Albumin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGA
Gene Full NameAspartylglucosaminidase
Alias SymbolsGA, AGU, ASRG
NCBI Gene Id175
Protein NameN(4)-(beta-N-acetylglucosaminyl)-L-asparaginase isoform 1 preproprotein
Description of TargetThis gene encodes a member of the N-terminal nucleophile (Ntn) hydrolase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta chains that comprise the mature enzyme. This enzyme is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. Mutations in this gene are associated with the lysosomal storage disease aspartylglycosaminuria that results in progressive neurodegeneration. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is subject to proteolytic processing..
Uniprot IDP20933
Protein Accession #NP_000018.2
Nucleotide Accession #NM_000027.3
Protein Size (# AA)346
Molecular Weight37 KDa
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